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Boster Bio
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Image Search Results
Journal: Journal of Biological Chemistry
Article Title: Silencing of Insulin-like Growth Factor-binding Protein-2 in Human Glioblastoma Cells Reduces Both Invasiveness and Expression of Progression-associated Gene CD24
doi: 10.1074/jbc.m609567200
Figure Lengend Snippet: FIGURE 2. Modulation of IGF/IGF-IR signaling caused by knockdown of IGFBP-2. The IGFBP-2-knockdown cells (IGFBP-2 KD) and control cells were treated with the indicated concentrations of IGF-I or IGF-II in serum-free con- dition, and extents of phosphorylation of IGF-IR (p-IGF-IR), Akt (p-Akt), and ERK1/2 (p-Erk1/2) were analyzed by immunoblot and a representative result of U251 was shown. The band intensity was measured and the ratio of phos- phorylated protein to the corresponding total protein was calculated.
Article Snippet: The following primary antibodies were used: anti-human IGFBP-2 mouse monoclonal antibody (C-10; Santa Cruz Biotechnology, Santa Cruz, CA), anti- -actinmousemonoclonal antibody (AC-74; Sigma), anti-phosphoERK1/2 rabbit monoclonal antibody (Thr185/Tyr187, clone AW39;Upstate Cell Signaling Solutions, Lake Placid, NY),
Techniques: Knockdown, Control, Phospho-proteomics, Western Blot
Journal: The Journal of Physiology
Article Title: Isolating the effects of carbohydrate and lipid availability on exercise‐induced skeletal muscle signalling in males
doi: 10.1113/JP289864
Figure Lengend Snippet: Fold change of phosphorylation status and total protein content pre‐ to post‐exercise of INSR Tyr1150/1151 , Akt Ser473 , AS160 Thr642 , GLUT4, AMPK Thr172 , ACC Ser79 , PDK4, PDH Ser293 and HSL Ser660 ( B ) with representative blots ( A ). Data shown as the mean ± SD. Differences between FAST vs . CARB and CARB vs . NIACIN assessed using a paired t test ( n = 8 males).
Article Snippet: Membranes were washed again in TBS‐T and incubated in primary antibodies against phospho Ser 79 acetyl‐CoA carboxlyase (ACC) (Cell Signaling Technologies, Danvers, MA, USA), phospho Thr172 AMP‐dependent protein kinase (AMPK) (Cell Signaling Technologies), phospho‐Thr642 Akt substrate of 160 kDa (AS160) (Cell Signaling Technologies),
Techniques: Phospho-proteomics